HepG2 cells develop signs of riboflavin deficiency within 4 days of culture in riboflavin-deficient medium

Riboflavin deficiency with ocular signs: report of a case.

Case Report A married woman, 52 years old, first seen September 1, 1948, was complaining of epiphora and a slight irritation of both eyes, particularly the right, with some photophobia. The illness was of some three months' duration, though she said she had had recurrent attacks of " sore eyes " for many years. The present attack seemed to have started after a long traip journey. The visual acu...

Regulation of riboflavin-metabolizing enzymes in riboflavin deficiency.

FASS, STEVEN, AND RICHARD S. RIVLIN. Regulation of riboflavinmetabolizing enzymes in ribuj?auin de_ficiency. Am, J. Physiol. 217(4) : 988-99 1. 1969.-The effects of riboflavin deficiency in rats on the hepatic activities of flavokinase, FAD pyrophosphorylase, and FMN phosphatase, three enzymes involved in riboflavin metabolism, have been measured and compared to the hepatic concentrations of FA...

Hemopoiesis in riboflavin-deficient rats.

Riboflavin deficiency induces a significant change in proteomic profiles in HepG2 cells

Riboflavin deficiency is widespread in many regions over the world, especially in underdeveloped countries. In this study, we investigated the effects of riboflavin deficiency on protein expression profiles in HepG2 cells in order to provide molecular information for the abnormalities induced by riboflavin deficiency. HepG2 cells were cultured in media containing different concentrations of rib...

Application of Riboflavin-Deficient Rat Erythrocytes in the in Vitro Assay of Faba Bean Toxicity

Background: Faba bean toxicity (favism) is a common condition observed in our region. Objective: To develop a short and simple technique involving the use of riboflavin-deficient rats to test in vivo faba bean toxicity. Methods/Results: Sprague Dawley rats were maintained on a riboflavin-deficient diet and their vitamin B2 status was monitored by the assay of erythrocyte glutathione reductase (...